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The Tomato Brown Rugose Fruit Virus (ToBRFV) is affecting all varieties of tomatoes and is spreading
worldwide. NRGene partnered with Philoseed to develop HR (high resistant) varieties

Our high resistance source has been tested successfully by several seed companies!

NRGene now offers licensing for both Philoseed/NRGene and BASF | Nunhems ToBRFV HR traits,
providing seeds and DNA markers.

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Outstanding Results

Figure 1. Symptoms of ToBRFV on fruits of sensitive tomato plants and symptoms on tomato leaves

Symptoms of ToBRFV on fruits of sensitive tomato plants and symptoms on tomato leaves by resistance scale.

Disease severity index (DSI):
(0) no visible symptoms
(1) light mosaic pattern on the apical leaf
(2) severe mosaic pattern on the apical leaf
(3) very severe mosaic pattern

Figure 2. Manhattan plot of significant QTL region in Chromosome 2. Significance threshold level is 4.5 LOD score

Major QTL on Chromosome 2

  • A major contributor to the resistance trait
  • Introgression from wild tomato
  • Specific markers designed and validated
  • QTL with high statistical significance

Figure 3. Manhattan plot of significant QTL region in Chromosome 7. LOD score = 9

Minor QTL on chromosome 7

  • QTL required but not sufficient to confer high resistance
  • Specific markers were designed and validated
  • Genomic location not previously described (2Mbp QTL – patent pending) LOD score of ~9

Figure 4. ToBRFV severity by new resistance gene zygosity level. Genotyping results of a F2 population using the designed marker, named NR_ToBRFVr-4 KASP marker. Symptoms scores (DSI): (0) no visible symptoms; (1) light mosaic pattern on the apical leaf; (2) severe mosaic pattern on the apical leaf; (3) very severe mosaic pattern

Genomic Analysis Results
Unique HR Gene Discovery

  • NRGene identified a novel TM1-like gene, absent in tomato reference genomes
  • The gene resulted from a duplication event downstream of the known TM1 gene
  • Specific markers were developed to differentiate it from existing TM1 alleles

 

To explore the discovery and development of the offered ToBRFV HR trait, including performance test result, download our white paper: 

Trait Characteristics

 

  • Phenotypes – no disease phenotypes on the fruit and leaves
  • Symptom free – for the whole season
  • IP protected

 

  • NON – GMO
  • Checked on different background
  • Suitable for all tomato segments

Package Includes

  • picon1

    SEEDS HARBORING THE TRAIT

  • picon2

    Marker for Marker-Assisted Selection

  • picon3

    Optional: Marker-Asisted BackCross
    Service by NRGene

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FREQUENTLY ASKED QUESTIONS

Is the resistance trait covered by recent patent applications from a third party?

No, the ToBRFV resistance trait was co-developed with Philoseed and an independent IP protection claim has been filed.

What tomato genetic background was used for the trait introgression?

The background used for the introgression process is a cultivated line for the big fresh tomato sector, indeterminate type.

Is linkage drag associated with the resistance?

No linkage drag has been observed for any obvious phenotypes including male sterility or plant and fruit size

Thanks for your interest in our product!

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